通过离心增强蓝舌病毒在细胞培养中的感染性。
Enhanced infectivity of bluetongue virus in cell culture by centrifugation.
作者信息
Sundin D R, Mecham J O
机构信息
Arthropod-Borne Animal Diseases Research Laboratory, U.S. Department of Agriculture, Laramie, Wyoming 82071-3965.
出版信息
J Clin Microbiol. 1989 Jul;27(7):1659-60. doi: 10.1128/jcm.27.7.1659-1660.1989.
The effects of centrifugation of the infection of cell culture with bluetongue virus (BTV) were investigated. Baby hamster kidney cells were infected with BTV with or without centrifugation. Viral antigen was detected by immunofluorescence at 24 h in both centrifuged and noncentrifuged cultures. However, after 24 h of infection, the production of PFU in centrifuged cell cultures was 10- to 20-fold greater than that seen in cultures not centrifuged. In addition, centrifugation enhanced the direct detection of PFU from blood samples collected from a sheep experimentally infected with BTV.
研究了用蓝舌病毒（BTV）感染细胞培养物时离心的效果。用或不用离心法使幼仓鼠肾细胞感染BTV。在离心和未离心的培养物中，24小时时通过免疫荧光检测病毒抗原。然而，感染24小时后，离心细胞培养物中蚀斑形成单位（PFU）的产生量比未离心培养物中高10至20倍。此外，离心增强了从实验感染BTV的绵羊采集的血样中直接检测PFU的能力。
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