Hudson J B
Division of Medical Microbiology, University of British Columbia, Vancouver, Canada.
J Virol Methods. 1988 Feb;19(2):97-108. doi: 10.1016/0166-0934(88)90153-x.
The phenomenon of centrifugal enhancement of infectivity, previously shown to be an intrinsic property of murine and human cytomegalovirus (CMV) was studied further in an attempt to understand the underlying mechanism of action and to try to influence the phenomenon by means of extrinsic factors. The sand-rat herpes virus also displayed centrifugal enhancement in rat, mouse, and human cells. Further investigations were performed with murine CMV. The property of enhancement was not restricted to a specific phase of cell cycle. A variety of factors, known to influence cellular physiology, did not significantly affect the ratio of centrifugal infectivity to standard infectivity i.e., the c/s ratio. However, it was necessary to centrifuge the infected cultures within one hour of the establishment of infection, since centrifugation after this delay failed to yield the enhancement; but there was no partial effect; the cultures either showed complete centrifugal enhancement or no such effect. Thus, it appears that some herpes viruses, during their initial encounter with susceptible cells, can be forced into preferential productive infection by the process of centrifugation, but after a lapse of an hour or more, centrifugation can no longer do this and most of the infected cells fail to replicate virus. Consequently we continue to recommend low speed centrifugation (1000 x g, for 30 min) in order to enhance CMV detection and to optimize infectivity assays.
感染性离心增强现象，先前已表明是鼠类和人类巨细胞病毒（CMV）的一种内在特性，为了理解其潜在作用机制并尝试通过外在因素影响该现象，我们对此进行了进一步研究。沙鼠疱疹病毒在大鼠、小鼠和人类细胞中也表现出离心增强现象。我们用鼠类CMV进行了进一步研究。增强特性并不局限于细胞周期的特定阶段。已知的多种影响细胞生理学的因素，并未显著影响离心感染性与标准感染性的比值，即c/s比值。然而，在感染确立后的一小时内对感染培养物进行离心是必要的，因为在此延迟后离心无法产生增强效果；而且不存在部分效应；培养物要么表现出完全的离心增强，要么没有这种效应。因此，似乎一些疱疹病毒在初次接触易感细胞时，可通过离心过程被迫进入优先生产性感染，但在一小时或更长时间后，离心就不再能做到这一点，并且大多数感染细胞无法复制病毒。因此，我们继续推荐低速离心（1000×g，30分钟）以增强CMV检测并优化感染性测定。
