Gogoi Nayanmoni, Babu Punuri Jayasekhar, Mahanta Chandan, Bora Utpal
Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
Mater Sci Eng C Mater Biol Appl. 2015 Jan;46:463-9. doi: 10.1016/j.msec.2014.10.069. Epub 2014 Oct 24.
Here we report the synthesis of silver nanoparticles using ethanolic flower extract of Nyctanthes arbortristis, UVvisible spectra and TEM indicated the successful formation of silver nanoparticles. Crystalline nature of the silver nanoparticles was confirmed by X-ray diffraction. Fourier Transform Infra-Red Spectroscopy analysis established the capping of the synthesized silver nanoparticles with phytochemicals naturally occurring in the ethanolic flower extract of N. arbortristis. The synthesized silver nanoparticles showed antibacterial activity against the pathogenic strain of Escherichia coli MTCC 443. Furthermore, cytotoxicity of the silver nanoparticles was tested on mouse fibroblastic cell line (L929) and found to be non-toxic, which thus proved their biocompatibility. Antibacterial activity and cytotoxicity assay carried out in this study open up an important perspective of the synthesized silver nanoparticles.
在此，我们报道了使用夜花（Nyctanthes arbortristis）乙醇花提取物合成银纳米颗粒的过程，紫外可见光谱和透射电子显微镜表明成功形成了银纳米颗粒。X射线衍射证实了银纳米颗粒的晶体性质。傅里叶变换红外光谱分析表明，合成的银纳米颗粒被夜花乙醇花提取物中天然存在的植物化学物质所包覆。合成的银纳米颗粒对大肠杆菌MTCC 443致病菌株表现出抗菌活性。此外，在小鼠成纤维细胞系（L929）上测试了银纳米颗粒的细胞毒性，发现其无毒，从而证明了它们的生物相容性。本研究中进行的抗菌活性和细胞毒性测定为合成的银纳米颗粒开辟了一个重要的前景。
