LEIGC长链非编码RNA通过抑制上皮-间质转化在胃癌中发挥肿瘤抑制作用。
LEIGC long non-coding RNA acts as a tumor suppressor in gastric carcinoma by inhibiting the epithelial-to-mesenchymal transition.
作者信息
Han Yuehua, Ye Jun, Wu Dang, Wu Pin, Chen Zhigang, Chen Jian, Gao Shunliang, Huang Jian
机构信息
Cancer Institute, Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310009, China.
出版信息
BMC Cancer. 2014 Dec 11;14:932. doi: 10.1186/1471-2407-14-932.
BACKGROUND
Long non-coding RNAs have been shown to have critical regulatory roles in cancer biology. However, the contributions of lncRNAs to gastric cancer remain largely unknown.
METHODS
The differential expression of lncRNAs in gastric cancer and paired non-cancerous tissues were identified by microarray and validated using quantitative real-time PCR. Gastric samples from patients with gastric cancer were further analyzed for levels of a specifically downregulated lncRNA (termed as LEIGC).
RESULTS
We found that there were significantly lower levels of LEIGC expression in cancer tissue than in adjacent non-cancerous tissues in human gastric cancers (P < 0.01). Overexpression of LEIGC suppressed tumor growth and cell proliferation, and enhanced the sensitivity of gastric cancer cells to 5-fluorouracil (5-FU), whereas knockdown of LEIGC showed the opposite effect. We further demonstrated LEIGC functions by inhibiting the epithelial-to-mesenchymal transition (EMT) in gastric cancer.
CONCLUSIONS
Our data suggested that LEIGC is a tumor-suppressing lncRNA in gastric cancer, and led us to propose that lncRNAs may play important regulatory roles in cancer development and progression.
背景
长链非编码RNA已被证明在癌症生物学中具有关键的调控作用。然而，lncRNA对胃癌的作用在很大程度上仍不清楚。
方法
通过微阵列鉴定lncRNA在胃癌组织和配对的癌旁组织中的差异表达，并使用定量实时PCR进行验证。对胃癌患者的胃样本进一步分析一种特异性下调的lncRNA（称为LEIGC）的水平。
结果
我们发现，在人类胃癌中，癌组织中LEIGC的表达水平显著低于相邻的癌旁组织（P < 0.01）。LEIGC的过表达抑制肿瘤生长和细胞增殖，并增强胃癌细胞对5-氟尿嘧啶（5-FU）的敏感性，而敲低LEIGC则显示出相反的效果。我们通过抑制胃癌中的上皮-间质转化（EMT）进一步证明了LEIGC的功能。
结论
我们的数据表明LEIGC是胃癌中的一种肿瘤抑制性lncRNA，并使我们提出lncRNA可能在癌症发生和发展中发挥重要的调控作用。
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