Gall C M, Isackson P J
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
Science. 1989 Aug 18;245(4919):758-61. doi: 10.1126/science.2549634.
Nerve growth factor (NGF) produced by telencephalic neurons provides critical trophic support for cholinergic neurons of the basal forebrain. In situ hybridization and nuclease protection analyses demonstrate that limbic seizures dramatically increase the amount of messenger RNA for NGF in the neurons of the hippocampal dentate gyrus within 1 hour of seizure onset and in broadly distributed neocortical and olfactory forebrain neurons some hours later. The increased messenger RNA species is indistinguishable from messenger RNA for transcript B of the beta subunit of NGF from mouse submandibular gland. Thus, the expression of a known growth factor is affected by unusual physiological activity, suggesting one route through which trophic interactions between neurons in adult brain can be modified.
端脑神经元产生的神经生长因子（NGF）为基底前脑的胆碱能神经元提供关键的营养支持。原位杂交和核酸酶保护分析表明，边缘性癫痫发作在发作开始后1小时内会显著增加海马齿状回神经元中NGF信使RNA的量，数小时后在广泛分布的新皮质和嗅觉前脑神经元中也会增加。增加的信使RNA种类与小鼠下颌下腺NGFβ亚基转录本B的信使RNA无法区分。因此，一种已知生长因子的表达受异常生理活动影响，这提示了一条可改变成体脑中神经元间营养相互作用的途径。
