Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, 210098 Nanjing, China.
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, 210098 Nanjing, China.
Chemosphere. 2015 Feb;121:110-6. doi: 10.1016/j.chemosphere.2014.11.056. Epub 2014 Dec 12.
The objective of this work was to identify the optimal dose range for good anti-algal effect of linoleic acid (LA) sustained-release microspheres and investigate their impact on the antioxidant enzymes (super oxide dismutase, Catalase and Peroxidase) activity changes of Microcystis aeruginosa, as well as the production and release of microcystins (MCs). Based on measured changes in algal cell density and inhibitory ratio (IR), the optimal dose of LA microspheres was 0.3 g L(-1) with over 90% of IR in this study. The Chlorophyll a content and antioxidant enzymes activity in the LA microspheres group decreased markedly until beyond the minimal detection limit after 16 d and 9 d, respectively. In addition, LA microspheres demonstrated no significant impact on the extracellular release of MCs during the culturing period. The amount of intracellular microcystin-LR (MC-LR) per 10(6) algal cells in LA microspheres group was highest among all groups during the whole experimental process. Under the sustained stress of LA released from LA microspheres, the LA microspheres could decrease the production and release of algal toxins. There was no increase in the total amount of MC-LR in the algal cell culture medium. These indicated that LA sustained-release microspheres represent a high degree of ecological safety and their practical applications for the treatment of water undergoing algal blooms need further study.
本研究旨在确定亚油酸（LA）缓释微球发挥良好抑藻效果的最佳剂量范围，并考察其对铜绿微囊藻抗氧化酶（超氧化物歧化酶、过氧化氢酶和过氧化物酶）活性变化的影响，以及微囊藻毒素（MCs）的产生和释放。基于藻细胞密度和抑制率（IR）的测量变化，LA 微球的最佳剂量为 0.3 g L(-1)，在本研究中超过 90%的 IR。LA 微球组的叶绿素 a 含量和抗氧化酶活性分别在 16 d 和 9 d 后明显下降至低于最小检测限。此外，LA 微球在培养期间对 MCs 的胞外释放没有显著影响。在整个实验过程中，LA 微球组每 10(6)个藻细胞中的细胞内微囊藻毒素-LR（MC-LR）含量在所有组中均最高。在 LA 从 LA 微球持续释放的持续胁迫下，LA 微球可以降低藻毒素的产生和释放。藻细胞培养液中 MC-LR 的总量没有增加。这表明 LA 缓释微球具有高度的生态安全性，其在处理藻类水华方面的实际应用需要进一步研究。
