Morein B, Belák S, Soós T, Rusvai M, McGwire B S, Bognár K
Department of Virology, National Veterinary Institute, Uppsala, Sweden.
Vet Microbiol. 1989 Jun;20(2):143-54. doi: 10.1016/0378-1135(89)90037-0.
An immunostimulating complex (iscom) containing the envelope proteins of pseudorabies virus (PRV) was prepared and its efficacy was evaluated in two experiments on sheep. In the first experiment, sheep were intramuscularly (i.m.) or intradermally (i.d.) vaccinated with PRV iscom doses varying between 1 and 81 micrograms. The vaccination was repeated on Day 21 and the animals were exposed to challenge infection by subcutaneous inoculation of 1000 TCID50 of the virulent Phylaxia strain on Day 35 after first vaccination. In the second experiment, sheep were i.m. vaccinated with single doses of iscom varying between 1 and 27 micrograms and challenge-infected on Day 14. It was found that: (1) the i.d. administration of PRV iscom has no advantage over i.m. administration (2); a single dose of greater than or equal to 3 micrograms of PRV iscom provided protection against the disease. In immunoblots, viral proteins of molecular masses 120, 109, 55, 53 and 32 kDa were detected with the sera obtained from iscom-vaccinated and subsequently challenge-infected sheep, but not with sera from sheep which were iscom-vaccinated only. The above findings indicated that: (1) by using iscom technology, potent subunit vaccines can be prepared to prevent Aujeszky's disease; (2) the selective incorporation of viral envelope proteins into iscoms gives the opportunity to discriminate between iscom-vaccinated and naturally infected animals.
制备了一种含有伪狂犬病病毒（PRV）包膜蛋白的免疫刺激复合物（iscom），并在两项绵羊实验中评估了其效力。在第一个实验中，给绵羊肌肉注射（i.m.）或皮内注射（i.d.）剂量在1至81微克之间变化的PRV iscom。在第21天重复接种疫苗，并在首次接种疫苗后第35天通过皮下接种1000 TCID50的强毒株Phylaxia对动物进行攻毒感染。在第二个实验中，给绵羊肌肉注射单剂量在1至27微克之间变化的iscom，并在第14天进行攻毒感染。结果发现：（1）PRV iscom皮内给药并不比肌肉注射有优势；（2）单剂量大于或等于3微克的PRV iscom可提供疾病保护。在免疫印迹中，用从接种iscom并随后攻毒感染的绵羊获得的血清检测到分子量为120、109、55、53和32 kDa的病毒蛋白，但仅接种iscom的绵羊血清未检测到。上述结果表明：（1）通过使用iscom技术，可以制备有效的亚单位疫苗来预防奥耶斯基氏病；（2）病毒包膜蛋白选择性掺入iscom中为区分接种iscom的动物和自然感染的动物提供了机会。
