Marlett J A, Chesters J G, Longacre M J, Bogdanske J J
Department of Nutritional Sciences, University of Wisconsin-Madison 53706.
Am J Clin Nutr. 1989 Sep;50(3):479-85. doi: 10.1093/ajcn/50.3.479.
The effects of different methods on the distribution of total neutral sugars (TNS), uronic acids (UA), and beta-glucans (beta G) between the soluble (S) and insoluble (I) fractions of dietary fiber (DF) were determined for peas, kidney beans, oat bran, rice, and macaroni. Incorporation of a protease step into the Theander method "A" modestly increased, and addition of a pepsin digestion further increased the proportion of total fiber recovered in the S fraction. The effect of extraction method on the distribution of TNS, UA, and beta G between the S and I fractions varied with the food. The three methods measured the same total DF in a food and 1-3% starch in the I fraction of peas and kidney beans. Use of dimethyl sulfoxide to solubilize starch, or elevated temperature to extract S components, had no effect on the distribution of DF between S and I fractions of peas and macaroni. Incomplete protein hydrolysis did not always lower Klason lignin and excluding lignin from the fiber complex did not always substantially increase the S fraction.
测定了不同方法对豌豆、菜豆、燕麦麸、大米和通心粉膳食纤维（DF）可溶（S）部分和不溶（I）部分之间总中性糖（TNS）、糖醛酸（UA）和β-葡聚糖（βG）分布的影响。在Theander方法“A”中加入蛋白酶步骤适度增加了回收率，而添加胃蛋白酶消化进一步提高了S部分中回收的总纤维比例。提取方法对TNS、UA和βG在S和I部分之间分布的影响因食物而异。这三种方法测得的一种食物中的总膳食纤维相同，豌豆和菜豆的I部分中含有1-3%的淀粉。使用二甲基亚砜溶解淀粉或升高温度提取S成分，对豌豆和通心粉的S和I部分之间的膳食纤维分布没有影响。不完全的蛋白质水解并不总是会降低Klason木质素，而将木质素排除在纤维复合物之外也并不总是会大幅增加S部分。
