Lichon M J, James K W
University of Tasmania, Department of Plant Science, Hobart 7001, Australia.
J AOAC Int. 1996 Jan-Feb;79(1):54-61.
A modified version of the Englyst procedure for determination of dietary fiber and carbohydrate fractions of foods is described. The method is based on hydrolysis of separated fractions and capillary gas chromatographic (GC) analysis of derivatized monomer sugars, UV photometry of uronic acids, gravimetric determination of residue, and analysis of nitrogen content of the residue by using a C, H, N elemental analyzer. Modifications of fractionation, derivatization, and GC procedures are discussed. Rigorous sample preparation is required for reproducibility of 200 mg test portions. Contaminants interfering with chromatography must be minimized. Results for reference samples (wheat bran and Buerre Bosc pears) and white and whole meal bread agree with literature values.
本文描述了一种改良的恩格利斯特法，用于测定食物中的膳食纤维和碳水化合物组分。该方法基于对分离组分的水解、衍生化单糖的毛细管气相色谱（GC）分析、糖醛酸的紫外光度法、残渣的重量法测定以及使用碳、氢、氮元素分析仪对残渣氮含量的分析。讨论了分级分离、衍生化和气相色谱程序的改进。为了使200毫克测试份具有可重复性，需要进行严格的样品制备。必须将干扰色谱分析的污染物降至最低。参考样品（麦麸和博斯克梨）以及白面包和全麦面包的结果与文献值一致。
