Visedo-Gonzalez E, Dixon H B
Department of Biochemistry, University of Cambridge, U.K.
Biochem J. 1989 May 15;260(1):299-301. doi: 10.1042/bj2600299.
2-Aminoethylarsonic acid was tested for its ability to act as a substrate for ethanolamine-phosphate cytidylytransferase as a cytidylyl acceptor in place of ethanolamine phosphate. The expected product, like all mixed anhydrides of arsonic acids, should hydrolyse spontaneously with regeneration of the substrate analogue and CMP formation; such CMP production was observed. The limiting velocity with aminoethylarsonic acid is about 90% that with ethanolamine phosphate, and the Michaelis constant is below 20 mM.
对2-氨基乙胂酸作为乙醇胺磷酸胞苷酰转移酶的底物替代乙醇胺磷酸作为胞苷酰受体的能力进行了测试。预期产物与所有胂酸的混合酸酐一样，应自发水解，使底物类似物再生并形成CMP；观察到了这种CMP的产生。氨基乙胂酸的极限速度约为乙醇胺磷酸的90%，米氏常数低于20 mM。
