Gresser M J
J Biol Chem. 1981 Jun 25;256(12):5981-3.
Submitochondrial particles from beef heart mitochondria synthesize ADP-arsenate from ADP and arsenate when energized by succinate. The ADP-arsenate formed hydrolyzes rapidly and this is almost certainly the mechanism by which arsenate "uncouples" oxidative phosphorylation. When sufficient hexokinase is present, a substantial portion of the ADP-arsenate formed reacts with glucose to form glucose 6-arsenate and ADP. The glucose 6-arsenate thus formed hydrolyzes, at pH 7.5 and 30 degrees C, under the conditions used, with a rate constant of 5.5 X 10(-4) s-1 and is a substrate for glucose-6-phosphate dehydrogenase.
来自牛心线粒体的亚线粒体颗粒在琥珀酸提供能量时，能从ADP和砷酸盐合成ADP - 砷酸。形成的ADP - 砷酸会迅速水解，这几乎肯定是砷酸“解偶联”氧化磷酸化的机制。当存在足够的己糖激酶时，形成的大部分ADP - 砷酸会与葡萄糖反应形成6 - 砷酸葡萄糖和ADP。如此形成的6 - 砷酸葡萄糖在所用条件下，于pH 7.5和30℃水解，速率常数为5.5×10⁻⁴ s⁻¹，并且是6 - 磷酸葡萄糖脱氢酶的底物。
