气相色谱-质谱联用技术同时且经济高效地测定人血清和尿液中的乙二醇和乙醇酸用于急诊毒理学分析
Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS.
作者信息
Hložek Tomáš, Bursová Miroslava, Čabala Radomír
机构信息
Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic.
Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, Albertov 6, 128 43 Prague 2, Czech Republic.
出版信息
Clin Biochem. 2015 Feb;48(3):189-91. doi: 10.1016/j.clinbiochem.2014.12.002. Epub 2014 Dec 10.
OBJECTIVES
A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes.
DESIGN AND METHODS
Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min.
RESULTS
The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%).
CONCLUSION
The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid).
目的
开发并验证一种简单、经济高效且快速的气相色谱-质谱联用法（GC-MS），用于同时测定乙二醇、1,2-丙二醇和乙醇酸，以用于临床毒理学目的。
设计与方法
血清/尿液中的乙醇酸直接用氯甲酸异丁酯成功衍生化，同时二醇类物质用苯硼酸进行衍生化。整个样品制备过程在10分钟内完成。
结果
该测定法在50至5000 mgL(-1)范围内呈二次曲线关系，具有足够的准确度（96.3 - 105.8%）和精密度（CV≤8.9%）。
结论
该方法成功应用于定量急诊科患者血清中选定的化合物，结果与平行的气相色谱-火焰离子化检测法（GC-FID）测量结果相关性良好（乙二醇的R(2)为0.9933，乙醇酸的R(2)为0.9943）。
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