Fraser A D, MacNeil W
Toxicology Laboratory, Victoria General Hospital, Halifax, Nova Scotia, Canada.
J Toxicol Clin Toxicol. 1993;31(3):397-405. doi: 10.3109/15563659309000408.
Monitoring of individuals poisoned with ethylene glycol involves analysis of ethylene glycol in serum. The objective of this procedure was to validate a colorimetric and gas chromatographic procedure for glycolic acid in serum. The colorimetric procedure requires no sophisticated instrumentation and has been shown to be specific for glycolic acid. A gas chromatographic procedure has also been developed involving methyl derivatization of glycolic acid and the internal standard (propionic acid). These methods have been used for the analysis of serum specimens from ethylene glycol poisoned patients. Glycolic acid has been recognized as the major toxic agent in ethylene glycol poisoning but current methods available do not allow analysis in a clinically relevant turnaround time. These two procedures allow glycolic acid quantitation by procedures readily set up in most clinical toxicology laboratories.
对乙二醇中毒个体的监测涉及血清中乙二醇的分析。本程序的目的是验证一种用于血清中乙醇酸的比色法和气相色谱法。比色法不需要复杂的仪器，并且已证明对乙醇酸具有特异性。还开发了一种气相色谱法，涉及乙醇酸和内标（丙酸）的甲基衍生化。这些方法已用于分析乙二醇中毒患者的血清样本。乙醇酸已被认为是乙二醇中毒的主要毒性物质，但目前可用的方法无法在临床相关的周转时间内进行分析。这两种方法可以通过大多数临床毒理学实验室容易建立的程序对乙醇酸进行定量。
