Yeon Ji-Yeong, Min Sung-Hun, Park Hyo-Jin, Kim Jin-Woo, Lee Yong-Hee, Park Soo-Yong, Jeong Pil-Soo, Park Humdai, Lee Dong-Seok, Kim Sun-Uk, Chang Kyu-Tae, Koo Deog-Bon
Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk 712-714, Republic of Korea.
J Reprod Dev. 2015;61(2):81-9. doi: 10.1262/jrd.2014-070. Epub 2014 Dec 12.
Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effects of mitochondrial fission inhibitor, mdivi-1, on developmental competence and mitochondrial function of porcine embryos and primary cells. Presumptive porcine embryos were cultured in PZM-3 medium supplemented with mdivi-1 (0, 10 and 50 μM) for 6 days. Porcine fibroblast cells were cultured in growth medium with mdivi-1 (0 and 50 μM) for 2 days. Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < 0.05). Moreover, loss of mitochondrial membrane potential in the mdivi-1 (50 μM) treated group was increased relative to the control group (P < 0.05). Subsequent evaluation revealed that the intracellular levels of reactive oxygen species (ROS) and the apoptotic index were increased by mdivi-1 (50 μM) treatment (P < 0.05). Finally, the expression of mitochondrial fission-related protein (Drp 1) was lower in the embryos and cells in the mdivi-1-treated group than the control group. Taken together, these results indicate that mdivi-1 treatment may inhibit developmental competence and mitochondrial function in porcine embryos and primary cells.
线粒体是高度动态的细胞器，会不断进行融合/裂变以及由大型动力蛋白相关GTP酶协调的活动。这些动态的线粒体过程会影响线粒体的形态、大小和功能。因此，本研究旨在评估线粒体裂变抑制剂mdivi-1对猪胚胎和原代细胞的发育能力及线粒体功能的影响。将假定的猪胚胎在添加了mdivi-1（0、10和50 μM）的PZM-3培养基中培养6天。将猪成纤维细胞在含有mdivi-1（0和50 μM）的生长培养基中培养2天。我们的结果表明，mdivi-1（50 μM）处理组的囊胚产生率和细胞生长率低于对照组（P < 0.05）。此外，mdivi-1（50 μM）处理组的线粒体膜电位丧失相对于对照组有所增加（P < 0.05）。后续评估显示，mdivi-1（50 μM）处理会增加细胞内活性氧（ROS）水平和凋亡指数（P < 0.05）。最后，mdivi-1处理组胚胎和细胞中线粒体裂变相关蛋白（Drp 1）的表达低于对照组。综上所述，这些结果表明mdivi-1处理可能会抑制猪胚胎和原代细胞的发育能力及线粒体功能。
