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过江藤属微卫星标记的种间转移性

Cross-species transferability of microsatellite markers in the genus Lippia.

作者信息

Santos C P, Rocha D S, Bajay M M, Santos F R C, Campos J B, Pinheiro J B, Zucchi M I, Silva-Mann R, Arrigoni-Blank M F, Blank A F

机构信息

Programa de Pós-Graduação em Biotecnologia de Recursos Naturais, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Laboratório de Biologia Molecular, Agência Paulista de Tecnologia dos Agronegócios, Polo Centro Sul, Piracicaba, SP, Brasil.

出版信息

Genet Mol Res. 2014 Nov 27;13(4):9846-50. doi: 10.4238/2014.November.27.11.

DOI:10.4238/2014.November.27.11
PMID:25501193
Abstract

The cross-species transferability of 20 microsatellite markers was tested in the genus Lippia. Eleven markers were polymorphic after screening 19 accessions of Lippia sidoides and Lippia gracilis maintained in the Active Germplasm Bank (AGB) from Universidade Federal de Sergipe. Additionally, 40 accessions of Lippia spp were collected in Sergipe to increase the germplasm bank. A total of 23, 22, and 36 alleles were identified, with an average of 2.3, 2.2, and 3.27 alleles per locus, respectively, for each group. The markers that were used were efficient tools to access genetic diversity in the germplasm bank and will be useful for further research aiming at the conservation and management of these important aromatic species.

摘要

在过江藤属植物中测试了20个微卫星标记的跨物种转移性。对塞尔希培联邦大学活性种质库(AGB)中保存的19份拟过江藤和细叶过江藤种质进行筛选后,有11个标记具有多态性。此外,在塞尔希培收集了40份过江藤属植物种质以扩充种质库。每组分别共鉴定出23、22和36个等位基因,每个位点平均分别有2.3、2.2和3.27个等位基因。所使用的标记是评估种质库中遗传多样性的有效工具,将有助于进一步开展旨在保护和管理这些重要芳香植物物种的研究。

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