广藿香（唇形科）微卫星引物的开发与特性分析

Development and characterization of microsatellite primers in Pogostemon cablin (Lamiaceae).

作者信息

Sandes S S, Pinheiro J B, Zucchi M I, Monteiro M, Arrigoni-Blank M F, Blank A F

机构信息

Laboratório de Cultura de Tecidos e Melhoramento Vegetal, Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

出版信息

Genet Mol Res. 2013 Aug 8;12(3):2837-40. doi: 10.4238/2013.August.8.4.

DOI:10.4238/2013.August.8.4

PMID:24065640

Abstract

Microsatellite primers were developed and optimized for patchouli (Pogostemon cablin) to characterize the patchouli Active Germplasm Bank of Universidade Federal de Sergipe. Creation of a genomic library for patchouli enabled the design of 12 microsatellite primers. Six of these microsatellites were polymorphic, revealing two well-defined groups of individuals that possess exclusive alleles. The data allowed us to characterize the patchouli active Germplasm Bank, identify its genetic diversity, and provide new information for researching this species.

摘要

为广藿香（Pogostemon cablin）开发并优化了微卫星引物，以对塞尔希培联邦大学的广藿香活性种质库进行特征描述。构建广藿香基因组文库使得能够设计出12对微卫星引物。其中6个微卫星具有多态性，揭示出两组明确的个体，它们拥有独特的等位基因。这些数据使我们能够对广藿香活性种质库进行特征描述，识别其遗传多样性，并为该物种的研究提供新信息。