香科植物粘毛香茶菜新型微卫星标记的开发与特性分析

Development and characterization of novel microsatellite markers in Hyptis pectinata (Lamiaceae).

作者信息

Blank A F, Jesus A S, Santos C P, Grando C, Pinheiro J B, Zucchi M I, Arrigoni-Blank M F

机构信息

Programa de Pós-Graduação em Biotecnologia de Recursos Naturais, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil

Programa de Pós-Graduação em Biotecnologia de Recursos Naturais, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

出版信息

Genet Mol Res. 2014 Dec 4;13(4):10173-6. doi: 10.4238/2014.December.4.11.

DOI:10.4238/2014.December.4.11

PMID:25501228

Abstract

A microsatellite-enriched library was constructed and a set of 19 SSR markers were developed to characterize a germplasm collection of Hyptis pectinata (L.) Poit., maintained at the Universidade Federal de Sergipe (UFS). Fifteen markers of 19 ranged from moderately to highly polymorphic. A total of 113 alleles were identified, with a mean of 7.52 alleles per locus. The mean HO and HE were 0.582 and 0.657, respectively. The primers developed were efficient tools for accessing the genetic diversity of the germplasm collection analyzed and may also be useful for other studies involving this species and other species in the genus Hyptis.

摘要

构建了一个富含微卫星的文库，并开发了一组19个SSR标记，用于表征保存在塞尔希培联邦大学（UFS）的具柄香科（Hyptis pectinata (L.) Poit.）种质资源库。19个标记中有15个具有中度至高多态性。共鉴定出113个等位基因，每个位点平均有7.52个等位基因。平均观察杂合度（HO）和期望杂合度（HE）分别为0.582和0.657。所开发的引物是分析种质资源库遗传多样性的有效工具，也可能对涉及该物种及香科属其他物种的其他研究有用。