迷迭香（Rosmarinus officinalis L.）水提取物对洋葱分生组织细胞的诱变和抗诱变作用。

Mutagenic and antimutagenic effects of aqueous extract of rosemary (Rosmarinus officinalis L.) on meristematic cells of Allium cepa.

作者信息

Felicidade I, Lima J D, Pesarini J R, Monreal A C D, Mantovani M S, Ribeiro L R, Oliveira R J

机构信息

Centro de Estudos em Nutrição e Genética Toxicológica, Centro Universitário Filadélfia, Londrina, PR, Brasil.

Centro de Estudos em Célula Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.

出版信息

Genet Mol Res. 2014 Nov 28;13(4):9986-96. doi: 10.4238/2014.November.28.3.

DOI:10.4238/2014.November.28.3

PMID:25501210

Abstract

Polyphenolic compounds present in rosemary were found to have antioxidant properties, anticarcinogenic activity, and to increase the detoxification of pro-carcinogens. The aim of the study was to determine the effect the aqueous extract of rosemary (AER) on mutagenicity induced by methylmethane sulfonate in meristematic cells of Allium cepa, as well as to describe its mode of action. Anti-mutagenicity experiments were carried out with 3 different concentrations of AER, which alone showed no mutagenic effects. In antimutagenicity experiments, AER showed chemopreventive activity in cultured meristematic cells of A. cepa against exposure to methylmethane sulfonate. Additionally, post-treatment and simultaneous treatment using pre-incubation protocols were the most effective. Evaluation of different protocols and the percent reduction in DNA indicated bioantimutagenic as well desmutagenic modes of action for AER. AER may be chemopreventive and antimutagenic.

摘要

人们发现迷迭香中含有的多酚类化合物具有抗氧化特性、抗癌活性，并能增强致癌物前体的解毒作用。本研究的目的是确定迷迭香水提取物（AER）对甲磺酸甲酯在洋葱分生细胞中诱导的致突变性的影响，并描述其作用方式。使用3种不同浓度的AER进行抗突变实验，这些浓度单独使用时均无致突变作用。在抗突变实验中，AER在洋葱培养的分生细胞中对暴露于甲磺酸甲酯表现出化学预防活性。此外，使用预孵育方案的后处理和同时处理最为有效。对不同方案和DNA减少百分比的评估表明，AER具有生物抗突变和抗诱变作用模式。AER可能具有化学预防和抗突变作用。