Center of Health Sciences (CCS), State University of Piauí (UESPI/FACIME), Department of Genetics, Laboratory of Genetics. Rua Olavo Bilac , 2335, 64.049-570 Teresina PI, Brazil.
Center of Natural Sciences (CCN), State University of Piauí, Laboratory of Genetics. Rua João Cabral , 2231, 64.002-150 Teresina PI, Brazil.
Biotech Histochem. 2020 Aug;95(6):464-473. doi: 10.1080/10520295.2020.1719197. Epub 2020 Feb 10.
(Tul.) L.P. Queiroz. (Fabaceae) traditionally is used in Brazilian medicine to treat catarrhal infections, diarrhea, hepatitis and anemia. We investigated the phytochemical profile, and mutagenicity and anti-mutagenicity of aqueous extracts of leaves of in cells and in mice. We investigated four concentrations of extract for the bioassay and three doses of extract for administration to mice. For the assay, we analyzed 5,000 meristematic cells to determine the mitotic index, mean number of chromosome alterations and percentage of damage reduction. For each animal assay, 2,000 normochromatic erythrocytes were evaluated per mouse to determine the number of micronuclei and the protective effect of the extract. Phytochemical analysis of the extract revealed reducing sugars, tannins and alkaloids, which likely did not interfere with the cell cycle of or cause damage to the DNA of or mice. The extract exhibited a protective effect in both organisms.
（图皮）L.P.奎罗斯（豆科）传统上被用于治疗巴西医学中的卡他性感染、腹泻、肝炎和贫血。我们研究了叶子的植物化学特征，以及水提物对细胞和小鼠的致突变性和抗突变性。我们为生物测定研究了四种浓度的提取物，为给小鼠给药研究了三种剂量的提取物。对于 测定，我们分析了 5000 个有丝分裂细胞以确定有丝分裂指数、染色体改变的平均数量和损伤减少的百分比。对于每个动物测定，每只小鼠评估了 2000 个正常染色体红细胞以确定微核数量和提取物的保护作用。提取物的植物化学分析显示出还原糖、单宁和生物碱，这可能不会干扰 细胞周期或导致 或小鼠的 DNA 损伤。提取物在两种生物体中都表现出保护作用。
