光叶榉多态性微卫星位点的分离与鉴定

Isolation and characterization of polymorphic microsatellite loci from Zelkova schneideriana Hand.-Mazz.

作者信息

Liu H L, Zhang R Q, Geng M L, Zhu J Y, Ma J L

机构信息

College of Forestry, Central South University of Forestry and Technology, Changsha, Hunan, China.

College of Forestry, Central South University of Forestry and Technology, Changsha, Hunan, China

出版信息

Genet Mol Res. 2014 Dec 3;13(4):10062-6. doi: 10.4238/2014.December.3.1.

DOI:10.4238/2014.December.3.1

PMID:25501217

Abstract

Zelkova schneideriana is a highly valued hardwood species. An improved technique for isolating codominant compound microsatellite markers was used to develop simple sequence repeat markers for Z. schneideriana. A total of 12 microsatellite loci were identified. Overall, the number of alleles per locus ranged from 8-19, with an average of 11.75. Observed heterozygosity and expected heterozygosity values ranged from 0.109-0.709 and 0.832-0.929, respectively. Polymorphic information content is from 0.803-0.915, with an average of 0.854. These markers will be very important for future research related to the genetic diversity, population structure, patterns of gene flow, and mating system of this species.

摘要

榉树是一种极有价值的硬木树种。采用一种改进的共显性复合微卫星标记分离技术来开发榉树的简单序列重复标记。共鉴定出12个微卫星位点。总体而言，每个位点的等位基因数在8至19个之间，平均为11.75个。观察到的杂合度和预期杂合度值分别在0.109至0.709和0.832至0.929之间。多态信息含量在0.803至0.915之间，平均为0.854。这些标记对于该物种未来有关遗传多样性、种群结构、基因流动模式和交配系统的研究将非常重要。