榉树（榆科）的从头转录组组装及EST-SSR标记开发
De novo transcriptome assembly and EST-SSR markers development for Zelkova schneideriana Hand.-Mazz. (Ulmaceae).
作者信息
Wang Lingdan, Zhang Riqing, Geng Maolin, Qin Yufeng, Liu Hailong, Li Lingli, Li Mimi
机构信息
Central South University of Forestry and Technology, Changsha, 410004 China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014 China.
出版信息
3 Biotech. 2021 Sep;11(9):420. doi: 10.1007/s13205-021-02968-5. Epub 2021 Aug 30.
UNLABELLED
Hand.-Mazz. of the Ulmaceae family is a Tertiary relict and economically deciduous tree species endemic to Central and Southern China. In this study, we performed a transcriptome sequencing of using high-throughput sequencing approach to detect polymorphic expressed sequence tag-simple sequence repeats (EST-SSR) markers. A total of 3,235 microsatellite loci were detected from 53,517 unigenes. A set of 30 microsatellite markers were randomly selected to validate in 41 individuals from three populations, of which 10 were polymorphic. The number of alleles per locus ranged from 3 to 11. The observed heterozygosity and expected heterozygosity ranged from 0.366 to 0.829 and 0.439 to 0.848, respectively. These polymorphic SSR primers showed good transferability across different species, and are valuable for future studies on genetic diversity, conservation, phylogeography, and species delimitation in , as well as other species.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-021-02968-5.
未标注
榆科的榉属植物是一种第三纪残遗植物，是中国中部和南部特有的经济落叶树种。在本研究中，我们采用高通量测序方法对其进行转录组测序，以检测多态性表达序列标签简单序列重复（EST-SSR）标记。从53,517个单基因中总共检测到3,235个微卫星位点。随机选择一组30个微卫星标记在来自三个种群的41个个体中进行验证，其中10个具有多态性。每个位点的等位基因数范围为3至11。观察到的杂合度和预期杂合度分别为0.366至0.829和0.439至0.848。这些多态性SSR引物在不同物种间显示出良好的转移性，对于未来榉属植物的遗传多样性、保护、系统地理学和物种界定以及其他榉属物种的研究具有重要价值。
补充信息
在线版本包含可在10.1007/s13205-021-02968-5获取的补充材料。
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