Yue Z, Rong J, Ping W, Bing Y, Xin Y, Feng L D, Yaping W
Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, China.
Department of Stomatology, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing, China.
Genet Mol Res. 2014 Dec 4;13(4):10086-96. doi: 10.4238/2014.December.4.3.
The elucidation of the molecular mechanisms underlying the effects of traditional Chinese medicines in clinical practice is a key step toward their worldwide application, and this topic is currently a subject of intense research interest. Rg1, a component of ginsenoside, has recently been shown to perform several pharmacological functions; however, the underlying mechanisms of these effects remain unclear. In the present study, we investigated whether Rg1 has an anti-senescence effect on hematopoietic stem cells (HSCs) and the possible molecular mechanisms driving any effects. The results showed that Rg1 could effectively delay tert-butyl hydroperoxide (t-BHP)-induced senescence and inhibit gene expression in the p16(INK4a)-Rb and p19(Arf)-p53-p21(Cip/Waf1) signaling pathways in HSCs. Our study suggested that these two signaling pathways might be potential targets for elucidating the molecular mechanisms of the Rg1 anti-senescence effect.
阐明中药在临床实践中发挥作用的分子机制是其在全球范围内应用的关键一步，目前这一课题是研究的热点。人参皂苷成分Rg1最近已被证明具有多种药理功能；然而，这些作用的潜在机制仍不清楚。在本研究中，我们研究了Rg1对造血干细胞（HSCs）是否具有抗衰老作用以及驱动任何作用的可能分子机制。结果表明，Rg1可有效延缓叔丁基过氧化氢（t-BHP）诱导的衰老，并抑制HSCs中p16（INK4a）-Rb和p19（Arf）-p53-p21（Cip/Waf1）信号通路中的基因表达。我们的研究表明，这两条信号通路可能是阐明Rg1抗衰老作用分子机制的潜在靶点。
