Li S G, Yan M Z, Zhang D, Ye M, Deng J J
Department of Geriatrics, The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang, Hubei, China
Department of Geriatrics, The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang, Hubei, China.
Genet Mol Res. 2016 Sep 2;15(3):gmr8409. doi: 10.4238/gmr.15038409.
The development of age-related cardiovascular disease is associated with the senescence of vascular cells. This study aimed to investigate the effect of ginsenoside Rg1 on vascular smooth muscle cell (VSMC) senescence. Primary VSMCs were cultured and divided into control, D-galactose (D-gal), Rg1-L, and Rg1-H groups, which were cultured without and with D-gal, and with low- and high-concentrations of Rg1, respectively. D-gal-induced cellular senescence was identified by b-galactosidase staining, and ultrastructural changes within the cells were observed. The expression of p16, p21, and p53 in the four groups of VSMCs was determined by western blotting, and the cell cycle was investigated by flow cytometry. Compared with the control group, there was an obvious change in the ultrastructure of VSMCs in the D-gal group, and the proportion of b-galactosidase-positive cells was significantly increased (P < 0.05). In addition, p16, p21, and p53 expression was significantly increased (P < 0.05) and the cell cycle was arrested in the G0/G1 phase. Compared with the D-gal group, the percentage of positive cells was significantly reduced (P < 0.05) in the Rg1 groups, the expression of p16, p21, and p53 was significantly reduced (P < 0.05), and the number of cells in the G0/G1 phase decreased (P < 0.05). Ginsenoside Rg1 can inhibit VSMC senescence, and the mechanisms may be related to its partial inhibition of the p16/Rb and p53-p21 signaling pathways during the cell cycle.
与年龄相关的心血管疾病的发展与血管细胞的衰老有关。本研究旨在探讨人参皂苷Rg1对血管平滑肌细胞（VSMC）衰老的影响。培养原代VSMC并将其分为对照组、D-半乳糖（D-gal）组、Rg1-L组和Rg1-H组，分别在不加和加入D-半乳糖的情况下，以及在低浓度和高浓度Rg1的条件下进行培养。通过β-半乳糖苷酶染色鉴定D-半乳糖诱导的细胞衰老，并观察细胞内的超微结构变化。通过蛋白质印迹法测定四组VSMC中p16、p21和p53的表达，并通过流式细胞术研究细胞周期。与对照组相比，D-gal组VSMC的超微结构有明显变化，β-半乳糖苷酶阳性细胞比例显著增加（P<0.05）。此外，p16、p21和p53表达显著增加（P<0.05），细胞周期停滞在G0/G1期。与D-gal组相比，Rg1组阳性细胞百分比显著降低（P<0.05），p16、p21和p53表达显著降低（P<0.05），G0/G1期细胞数量减少（P<0.05）。人参皂苷Rg1可抑制VSMC衰老，其机制可能与其在细胞周期中部分抑制p16/Rb和p53-p21信号通路有关。
