Naeem A, Khan A A, Cheema H M N, Khan I A, Buerkert A
Plant Genetic Resources Lab, University of Agriculture, Faisalabad, Pakistan.
Plant Genetic Resources Lab, University of Agriculture, Faisalabad, Pakistan
Genet Mol Res. 2014 Dec 4;13(4):10341-8. doi: 10.4238/2014.December.4.29.
DNA barcoding is a promising tool for species identification at the molecular level. The barcoding system is well established for species differentiation in animals, while it is less common in plants. We evaluated 2 barcoding regions, maturase K (matK) and ribulose bisphosphate carboxylase (rbcL), to compare species of Palmae according to amplification success, discrimination power, and inter- and intra-specific divergence. Both regions appear to have potential to discriminate most species of Palmae, but 2 species, Phoenix dactylifera and Phoenix sylvestris, did not show variation in the nucleotides of the barcode genes. P. sylvestris is said to be the sister species of P. dactilyfera according to its morphological and genetic proximity to the cultivated date palm. Thus, the status of these 2 species needs to be re-evaluated considering more genes as barcodes. Furthermore, rbcL has a higher discrimination power (90%) than matK (66.6%) and can thus be potentially used as a standard barcode to discriminate the species of Palmae.
DNA条形码是一种在分子水平上进行物种鉴定的很有前景的工具。条形码系统在动物物种鉴别方面已经很成熟，而在植物中则不太常见。我们评估了两个条形码区域，成熟酶K（matK）和核酮糖二磷酸羧化酶（rbcL），以根据扩增成功率、鉴别能力以及种间和种内差异来比较棕榈科物种。这两个区域似乎都有潜力鉴别大多数棕榈科物种，但有两个物种，即海枣和野生海枣，在条形码基因的核苷酸上没有显示出变异。根据其与栽培枣椰树在形态和遗传上的相近性，野生海枣被认为是海枣的姐妹物种。因此，需要考虑更多基因作为条形码来重新评估这两个物种的地位。此外，rbcL的鉴别能力（90%）高于matK（66.6%），因此有可能用作鉴别棕榈科物种的标准条形码。
