Jin Dong-Pil, Sim Sunhee, Park Jong-Won, Choi Ji-Eun, Yoon Jiwon, Lim Chae Eun, Kim Min-Ha
Urban Biodiversity Research Division, Sejong National Arboretum, Korean Arboretum and Gardens Institute, Sejong 30106, Republic of Korea.
Plant Resources Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Plants (Basel). 2023 May 22;12(10):2060. doi: 10.3390/plants12102060.
Caryophyllaceae is a large angiosperm family, with many species being utilized as ornamental or medicinal plants in Korea, in addition to several endangered species that are managed by the government. In this study, we used DNA barcoding for the accurate identification of Korean Caryophyllaceae. A total of 78 taxa ( = 215) were sequenced based on three chloroplast regions (L, K, and A-H) and nuclear ribosomal internal transcribed spacers (ITS). In the neighbor-joining tree, a higher accuracy of identification was generally observed when using ITS (>73%) rather than chloroplast regions (<62%). The highest resolution was found for L + ITS (77.6%), although resolution varied according to the genus. Among the genera that included two and more species, five genera (, , , , and ) were successfully identified. However, the species of five other genera (, , , , and ) showed relatively low resolutions (0-61.1%). In the cases of , , and , ambiguous taxonomic relationships among unidentified species may have been a factor contributing to such low resolutions. However, in contrast to these results, and have been identified well in previous studies. Our findings indicate the need of taxonomic reconsideration in Korea.
石竹科是一个庞大的被子植物科，在韩国，除了几种由政府管理的濒危物种外，许多物种被用作观赏植物或药用植物。在本研究中，我们使用DNA条形码技术对韩国石竹科植物进行准确鉴定。基于三个叶绿体区域（L、K和A-H）和核糖体内部转录间隔区（ITS），共对78个分类单元（=215个）进行了测序。在邻接树中，使用ITS（>73%）时的鉴定准确率通常高于叶绿体区域（<62%）。尽管分辨率因属而异，但L+ITS的分辨率最高（77.6%）。在包含两个及以上物种的属中，五个属（、、、和）被成功鉴定。然而，其他五个属（、、、和）的物种分辨率相对较低（0-61.1%）。在、和的情况下，未鉴定物种之间模糊的分类关系可能是导致这种低分辨率的一个因素。然而，与这些结果相反，和在先前的研究中已得到很好的鉴定。我们的研究结果表明，韩国需要重新进行分类学考量。
