Dombrádi V, Axton J M, Glover D M, Cohen P T
Department of Biochemistry, Medical Sciences Institute, The University, Dundee, Scotland.
Eur J Biochem. 1989 Aug 15;183(3):603-10. doi: 10.1111/j.1432-1033.1989.tb21089.x.
A 1.2-kb clone containing the full coding sequence of a protein phosphatase 1 catalytic subunit has been isolated from a Drosophila head cDNA library. It encodes a polypeptide of 302 amino acids with a molecular mass of 34.5 kDa. The predicted protein sequence is 92% identical (94% similar) to rabbit protein phosphatase 1 alpha (PP-1 alpha) demonstrating strict conservation of the phosphatase catalytic subunit over a considerable evolutionary distance. Abundant 1.6-kb and 2.5-kb mRNA transcripts were detected throughout Drosophila development. The clone hybridised to four sites on Drosophila salivary gland polytene chromosomes. The major site is at 87B6-12 on the right arm of chromosome 3. In addition, there are three secondary sites, one on the same chromosome at 96A2-5 and two on the X chromosome at 9C1-2 and 13C1-2. Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88% similar to Drosophila PP-1 alpha, proves the existence of at least two transcriptionally active genes for protein phosphatase 1.
从果蝇头部cDNA文库中分离出一个包含蛋白磷酸酶1催化亚基完整编码序列的1.2 kb克隆。它编码一个由302个氨基酸组成的多肽，分子量为34.5 kDa。预测的蛋白质序列与兔蛋白磷酸酶1α（PP - 1α）有92%的同一性（94%的相似性），这表明在相当长的进化距离上，磷酸酶催化亚基具有严格的保守性。在果蝇的整个发育过程中都检测到了丰富的1.6 kb和2.5 kb mRNA转录本。该克隆与果蝇唾液腺多线染色体上的四个位点杂交。主要位点位于3号染色体右臂的87B6 - 12处。此外，还有三个次要位点，一个在同一条染色体的96A2 - 5处，两个在X染色体的9C1 - 2和13C1 - 2处。另一个与9C1 - 2杂交并编码与果蝇PP - 1α 88%相似的催化亚基部分的cDNA克隆的分离，证明了至少存在两个蛋白磷酸酶1的转录活性基因。
