Department of Urology, Medical School, National & Kapodistian University of Athens, Athens, Greece
Department of Physiology, Medical School, National & Kapodistian University of Athens, Athens, Greece.
Anticancer Res. 2014 Dec;34(12):7415-24.
The aim of this study was to develop multiplex-PCR assays for the detection of circulating tumor cells in peripheral blood and urine samples of patients with bladder cancer.
Peripheral blood and urine samples were collected from 208 patients (169 patients and 39 healthy volunteers). After RNA extraction and cDNA synthesis, the samples were analyzed for the expression of cytokeratin 19 (CK19), CK20 and epidermal growth factor receptor (EGFR) mRNA in blood and for SURVIVIN, human telomerase reverse transcriptase (hTERT), cytokeratin 20 (CK20) mRNA in urine, using multiplex-PCR assays.
EGFR and CK20 alone or in combination as well as all urine markers correlated well with histological grade. hTERT correlated well with primary tumor size T≥3. Patients with positive urine markers had significantly worse progression-free survival.
Multiplex-PCR assays can be a useful tool for staging and monitoring purposes in patients with bladder cancer.
本研究旨在开发用于检测膀胱癌患者外周血和尿液样本中循环肿瘤细胞的多重 PCR 检测方法。
收集了 208 名患者（169 名患者和 39 名健康志愿者）的外周血和尿液样本。提取 RNA 并合成 cDNA 后，使用多重 PCR 检测方法分析血液中细胞角蛋白 19（CK19）、CK20 和表皮生长因子受体（EGFR）mRNA 的表达，以及尿液中 SURVIVIN、人端粒酶逆转录酶（hTERT）、CK20mRNA 的表达。
EGFR 和 CK20 单独或联合以及所有尿标志物与组织学分级密切相关。hTERT 与原发肿瘤大小 T≥3 密切相关。尿液标志物阳性的患者无进展生存期明显较差。
多重 PCR 检测方法可作为膀胱癌患者分期和监测的有用工具。
