使用多重 PCR 分析检测结直肠癌和胃癌的循环肿瘤细胞。
Detection of circulating tumor cells in colorectal and gastric cancer using a multiplex PCR assay.
机构信息
Department of Experimental Physiology, National and Kapodistrian University of Athens, Medical School, Athens, Greece
Second Department of Propedeutic Surgery, Laiko General Hospital, National and Kapodistrian University of Athens, Medical School, Athens, Greece.
出版信息
Anticancer Res. 2014 Jun;34(6):3083-92.
AIM
The aim of this study was the development of a multiplex-PCR assay for the detection of circulating tumor cells in patients with colorectal and gastric cancer.
PATIENTS AND METHODS
Peripheral blood samples were collected from 81 patients with colorectal cancer, 16 with gastric cancer and 38 healthy blood donors, as controls. The samples were processed for RNA extraction and cDNA synthesis and were subsequently analyzed for the expression of cytokeratin 19 (CK19), cytokeratin 20(CK20) and epidermal growth factor receptor (EGFR) with multiplex PCR.
RESULTS
Statistical analysis revealed that the combination of CK19 and CK20 could be useful in the exclusion of colorectal cancer, as well as the diagnosis and exclusion of gastric cancer. Furthermore, the expression of EGFR was correlated with the presence of systemic disease in patients with colorectal cancer.
CONCLUSION
Multiplex-PCR-based detection of circulating tumor cells could serve as a useful tool for the diagnosis, and monitoring of patients with colorectal and gastric cancer.
目的
本研究旨在开发一种用于检测结直肠癌和胃癌患者循环肿瘤细胞的多重 PCR 检测方法。
患者和方法
采集 81 例结直肠癌患者、16 例胃癌患者和 38 例健康献血者的外周血样本作为对照。对样本进行 RNA 提取和 cDNA 合成处理，然后使用多重 PCR 分析细胞角蛋白 19（CK19）、细胞角蛋白 20（CK20）和表皮生长因子受体（EGFR）的表达。
结果
统计分析表明，CK19 和 CK20 的联合检测可用于排除结直肠癌，以及诊断和排除胃癌。此外，EGFR 的表达与结直肠癌患者全身性疾病的存在相关。
结论
基于多重 PCR 的循环肿瘤细胞检测可作为一种有用的工具，用于结直肠癌和胃癌患者的诊断和监测。
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