Division of Histopathology and Cytopathology, Azienda Sanitaria Locale n° 5, La Spezia, Italy.
Division of Pneumology, Azienda Sanitaria Locale n° 5, La Spezia, Italy.
Anticancer Res. 2014 Dec;34(12):7425-9.
BACKGROUND/AIM: Mesothelin (SMRP) is regarded as a biomarker of malignant pleural mesothelioma (MPM). Herein, we analyzed the contribution of SMRP detection in pleural effusion and in serum to the diagnosis of MPM with non-positive cytology.
The present study included 52 cases of MPM, 43 of pleural benign lesions and 25 of non-MPM pleural metastases. SMRP was measured by MesoMark ELISA (Cis-Bio International Gif/Yvette; France).
In non-positive cytology, effusion-SMRP showed higher diagnostic performance than serum-SMRP. We found 38 out of 52 (73.1%) cases of non-positive cytology MPM, out of which 27 (71.0%) were positive for effusion-SMRP (cut-off=12.70 nM) and 18 (47.4%) for serum-SMRP (cut-off=1.08 nM). When cytology, effusion- and serum-SMRP were used in combination, an overall sensitivity in detection of MPM of 78.9% was achieved. The same sensitivity was obtained by combining cytology with effusion-SMRP alone, whereas the combination of serum-SMRP with cytology led to a sensitivity of 61.5%.
Detection of both effusion- and serum-SMRP can contribute to improve the diagnosis of MPM with non-positive cytology. However, the analysis of SMRP in effusion makes it unnecessary to test SMRP in the serum.
背景/目的：间皮素（SMRP）被认为是恶性胸膜间皮瘤（MPM）的生物标志物。在此，我们分析了胸腔积液和血清中 SMRP 检测对非阳性细胞学的 MPM 的诊断贡献。
本研究包括 52 例 MPM、43 例胸膜良性病变和 25 例非 MPM 胸膜转移。SMRP 通过 MesoMark ELISA（Cis-Bio International Gif/Yvette；法国）进行测量。
在非阳性细胞学中，胸腔积液-SMRP 比血清-SMRP 具有更高的诊断性能。我们发现 52 例非阳性细胞学 MPM 中有 38 例（73.1%），其中 27 例（71.0%）胸腔积液-SMRP 阳性（cut-off=12.70 nM），18 例（47.4%）血清-SMRP 阳性（cut-off=1.08 nM）。当细胞学、胸腔积液和血清-SMRP 联合使用时，对 MPM 的总检测敏感性为 78.9%。单独结合细胞学和胸腔积液-SMRP 也可获得相同的敏感性，而血清-SMRP 与细胞学联合使用的敏感性为 61.5%。
检测胸腔积液和血清中的 SMRP 均可有助于提高非阳性细胞学 MPM 的诊断。然而，分析胸腔积液中的 SMRP 使得检测血清中的 SMRP 变得不必要。
