Delahanty J N, Evans J C, Rowlands C C, Barratt M D, Pendlington R U
Department of Chemistry, University College, Cardiff, U.K.
Free Radic Biol Med. 1989;7(3):231-6. doi: 10.1016/0891-5849(89)90129-9.
Aqueous solutions (pH = 8) of both 3,3'-dimethyl and 4,4'-dimethyl substituted analogues of the photoallergen fentichlor (bis(2-hydroxy-5-chlorophenyl)sulphide) produced stable semiquinone radicals when irradiated with u.v. light (greater than 310 nm). These radicals have been characterised using electron spin resonance techniques: the results confirm the assignment of hyperfine coupling constants for the parent fentichlor radical. The binding of fentichlor to HSA was found to be partly oxygen dependent demonstrating a role for semiquinone type radicals in the binding mechanism. The stoichiometry and specificity of the binding of the dimethyl analogues to soluble proteins were found to be similar to that of fentichlor itself.
光变应原芬替氯（双（2-羟基-5-氯苯基）硫化物）的3,3'-二甲基和4,4'-二甲基取代类似物的水溶液（pH = 8）在紫外光（大于310 nm）照射下会产生稳定的半醌自由基。这些自由基已通过电子自旋共振技术进行了表征：结果证实了母体芬替氯自由基的超精细偶合常数的归属。发现芬替氯与HSA的结合部分依赖于氧气，这表明半醌型自由基在结合机制中起作用。发现二甲基类似物与可溶性蛋白质结合的化学计量和特异性与芬替氯本身相似。
