Barratt M D, Goodwin B F, Lovell W W
Environmental Safety Laboratory, Unilever Research, Sharnbrook, Bedford, UK.
Int Arch Allergy Appl Immunol. 1987;84(4):385-9. doi: 10.1159/000234454.
Photoconjugates were prepared by ultraviolet irradiation of guinea-pig albumin (GPA) with the photoallergens tetrachlorosalicylanilide (T4CS) and fentichlor. Injections of T4CS-GPA induced photoallergy to T4CS in 11 of 12 guinea-pigs whereas injections of fentichlor-GPA induced photoallergy in 5 of 12 guinea-pigs. Thus the fentichlor-GPA photoconjugate, which contained a molar ratio of hapten to protein 3 times higher than the corresponding T4CS conjugate, produced a significantly lower response. The results demonstrate the importance of protein conjugate formation in the induction of photoallergy, i.e. the role of carrier protein in contact sensitivity. The high potency of the T4CS-GPA photoconjugate in inducing photoallergy suggests that albumin may have a special role as a carrier protein in T4CS photoallergy.
通过用光变应原四氯水杨酰苯胺（T4CS）和芬替氯对豚鼠白蛋白（GPA）进行紫外线照射来制备光共轭物。注射T4CS-GPA可使12只豚鼠中的11只对T4CS产生光变应性，而注射芬替氯-GPA可使12只豚鼠中的5只产生光变应性。因此，芬替氯-GPA光共轭物中半抗原与蛋白质的摩尔比比相应的T4CS共轭物高3倍，但其产生的反应明显较低。结果证明了蛋白质共轭物形成在光变应性诱导中的重要性，即载体蛋白在接触性过敏反应中的作用。T4CS-GPA光共轭物诱导光变应性的高效力表明白蛋白在T4CS光变应性中可能作为载体蛋白发挥特殊作用。
