Hensler T, Raulf M, Megret F, Alouf J E, König W
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Federal Republic of Germany.
Infect Immun. 1989 Oct;57(10):3165-71. doi: 10.1128/iai.57.10.3165-3171.1989.
The purpose of our study was to characterize the properties of the interaction of pertussis toxin with human polymorphonuclear leukocytes for the modulation of leukotriene generation and metabolism. The cells were stimulated with either the Ca ionophore A23187, opsonized zymosan, or the bacterial peptide formyl-methionyl-leucyl phenylalanine. Incubation of the cells with pertussis toxin led to a rapid inhibition of LTB4 generation when formyl-methionyl-leucyl phenylalanine was used as the stimulus, whereas there was no effect with the Ca ionophore and just a low effect with opsonized zymosan. The inhibition of leukotriene generation was dependent on the incubation time, temperature, and pertussis toxin concentration. The effect was not dependent on the presence of calcium. Incubation of the cells with guanosine 5'-O-(3-thiotriphosphate) the stable analog of GTP, led to a time-dependent increase in leukotriene generation induced by formyl-methionyl-leucyl phenylalanine which was abolished by the simultaneous addition of pertussis toxin. Our data suggest that the formyl-methionyl-leucyl phenylalanine-induced generation of leukotrienes is dependent on a GTP-binding protein. The participation of the various G proteins has yet to be elucidated.
我们研究的目的是描述百日咳毒素与人多形核白细胞相互作用的特性，以调节白三烯的生成和代谢。用钙离子载体A23187、调理酵母聚糖或细菌肽甲酰甲硫氨酰亮氨酰苯丙氨酸刺激细胞。当使用甲酰甲硫氨酰亮氨酰苯丙氨酸作为刺激物时，将细胞与百日咳毒素一起孵育会导致LTB4生成迅速受到抑制，而钙离子载体则无此作用，调理酵母聚糖的作用较弱。白三烯生成的抑制取决于孵育时间、温度和百日咳毒素浓度。该作用不依赖于钙的存在。将细胞与GTP的稳定类似物鸟苷5'-O-(3-硫代三磷酸)一起孵育，会导致甲酰甲硫氨酰亮氨酰苯丙氨酸诱导的白三烯生成随时间增加，而同时添加百日咳毒素可消除这种增加。我们的数据表明，甲酰甲硫氨酰亮氨酰苯丙氨酸诱导的白三烯生成依赖于一种GTP结合蛋白。各种G蛋白的参与情况尚待阐明。
