Cook Kate B, Hughes Timothy R, Morris Quaid D
Brief Funct Genomics. 2015 Jan;14(1):74-89. doi: 10.1093/bfgp/elu047. Epub 2014 Dec 13.
RNA-binding proteins (RBPs) are important regulators of eukaryotic gene expression. Genomes typically encode dozens to hundreds of proteins containing RNA-binding domains, which collectively recognize diverse RNA sequences and structures. Recent advances in high-throughput methods for assaying the targets of RBPs in vitro and in vivo allow large-scale derivation of RNA-binding motifs as well as determination of RNA-protein interactions in living cells. In parallel, many computational methods have been developed to analyze and interpret these data. The interplay between RNA secondary structure and RBP binding has also been a growing theme. Integrating RNA-protein interaction data with observations of post-transcriptional regulation will enhance our understanding of the roles of these important proteins.
RNA结合蛋白（RBPs）是真核基因表达的重要调节因子。基因组通常编码数十到数百种含有RNA结合结构域的蛋白质，这些蛋白质共同识别各种RNA序列和结构。用于在体外和体内测定RBPs靶标的高通量方法的最新进展，使得能够大规模推导RNA结合基序以及确定活细胞中的RNA-蛋白质相互作用。与此同时，已经开发了许多计算方法来分析和解释这些数据。RNA二级结构与RBP结合之间的相互作用也一直是一个日益受到关注的主题。将RNA-蛋白质相互作用数据与转录后调控的观察结果相结合，将增进我们对这些重要蛋白质作用的理解。
