Department of Chemistry-BMC, Uppsala University, Box 576, Uppsala, 75123 (Sweden).
Angew Chem Int Ed Engl. 2015 Feb 2;54(6):1787-90. doi: 10.1002/anie.201408560. Epub 2014 Dec 11.
Multiplex analyte detection in complex dynamic systems is desirable for the investigation of cellular communication networks as well as in medical diagnostics. A family of lanthanide-based responsive luminescent probes for multiplex detection is reported. The high modularity of the probe design enabled the rapid assembly of both green and red emitters for a large variety of analytes by the simple exchange of the lanthanide or an analyte-cleavable caging group, respectively. The real-time three-color detection of up to three analytes was demonstrated, thus setting the stage for the non-invasive investigation of interconnected biological processes.
在复杂动态系统中进行多重分析物检测对于研究细胞通讯网络以及医学诊断都是非常有意义的。本文报道了一种基于镧系元素的响应性发光探针家族，用于多重检测。该探针设计具有很高的模块性，可以通过简单地交换镧系元素或可分析物切割笼基团，快速组装出各种绿色和红色发射器，从而对多种分析物进行检测。该探针实现了实时三通道检测，为非侵入式研究相互关联的生物过程奠定了基础。
