Liu Xin Chao, Liu Qiyong, Chen Xu Bo, Zhou Ligang, Liu Zhi Long
Department of Entomology, China Agricultural University, Haidian District, Beijing, China.
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China.
Pest Manag Sci. 2015 Nov;71(11):1582-6. doi: 10.1002/ps.3964. Epub 2015 Jan 14.
In our screening programme for new agrochemicals from wild plants, the essential oil of Tetradium glabrifolium (Champ. ex Benth.) T.G. Hartley fruits was found to possess strong larvicidal activity against the Asian tiger mosquito, Aedes albopictus L. The essential oil was extracted via hydrodistillation, and the constituents were determined by GC-MS analysis. The active compounds were isolated and identified by bioassay-directed fractionation.
GC-MS analyses revealed the presence of 19 components with 2-tridecanone (43.38%), 2-undecanone (24.09%), D-limonene (13.01%), caryophyllene (5.04%) and β-elemene (4.07%) being the major constituents. Bioactivity-directed chromatographic separation of the oil led to the isolation of 2-tridecanone, 2-undecanone and D-limonene as active compounds. The essential oil of T. glabrifolium exhibited larvicidal activity against the early fourth-instar larvae of A. albopictus, with an LC50 value of 8.20 µg mL(-1). The isolated constituent compounds, 2-tridecanone, 2-undecanone and D-limonene, possessed strong larvicidal activity against the early fourth-instar larvae of A. albopictus, with LC50 values of 2.86, 9.95 and 41.75 µg mL(-1) respectively.
The findings indicated that the essential oil of T. glabrifolium fruits and the three constituents have an excellent potential for use in control of A. albopictus larvae and could be useful in the search for newer, safer and more effective natural compounds as larvicides.
在我们从野生植物中筛选新型农用化学品的项目中，发现光叶吴茱萸（Tetradium glabrifolium (Champ. ex Benth.) T.G. Hartley）果实的精油对白纹伊蚊（Aedes albopictus L.）具有很强的杀幼虫活性。通过水蒸馏法提取该精油，并采用气相色谱 - 质谱联用（GC - MS）分析确定其成分。通过生物活性导向分级分离法分离并鉴定了活性化合物。
GC - MS分析显示存在19种成分，其中2 - 十三烷酮（43.38%）、2 - 十一烷酮（24.09%）、D - 柠檬烯（13.01%）、石竹烯（5.04%）和β - 榄香烯（4.07%）为主要成分。对该精油进行生物活性导向的色谱分离，得到了2 - 十三烷酮、2 - 十一烷酮和D - 柠檬烯作为活性化合物。光叶吴茱萸精油对白纹伊蚊早期四龄幼虫表现出杀幼虫活性，LC50值为8.20 μg mL(-1)。分离得到的成分化合物2 - 十三烷酮、2 - 十一烷酮和D - 柠檬烯对白纹伊蚊早期四龄幼虫具有很强的杀幼虫活性，LC50值分别为2.86、9.95和41.75 μg mL(-1)。
研究结果表明，光叶吴茱萸果实的精油及其三种成分在控制白纹伊蚊幼虫方面具有极好的应用潜力，并且在寻找更新、更安全、更有效的天然杀幼虫化合物方面可能会有所帮助。
