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评价薤白精油及其选定的主要成分化合物对白纹伊蚊(双翅目:蚊科)的杀幼虫活性。

Evaluation of larvicidal activity of the essential oil of Allium macrostemon Bunge and its selected major constituent compounds against Aedes albopictus (Diptera: Culicidae).

机构信息

Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China.

出版信息

Parasit Vectors. 2014 Apr 15;7:184. doi: 10.1186/1756-3305-7-184.

Abstract

BACKGROUND

During the screening programme for new agrochemicals from Chinese medicinal herbs and local wild plants, the essential oil of dried bulbs of Allium macrostemon Bunge (Liliaceae) was found to possess larvicidal activity against mosquitoes. The aim of this research was to determine the larvicidal activity of the essential oil and its major constituent compounds against the larvae of the Culicidae mosquito, Aedes albopictus.

METHODS

Essential oil of A. macrostemon was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mass spectrometry (GC-MS). The activity of the essential oil and its two major constituents were evaluated, using World Health Organization (WHO) procedures, against the fourth instar larvae of Ae. albopictus for 24 h and larval mortalities were recorded at various essential oil/compound concentrations ranging from 9.0 - 150 μg/ml.

RESULTS

The essential oil of A. macrostemon exhibited larvicidal activity against the early fourth instar larvae of Ae. albopictus with an LC₅₀ value of 72.86 μg/ml. The two constituent compounds, dimethyl trisulfide and methyl propyl disulfide possessed strong larvicidal activity against the early fourth instar larvae of Ae. albopictus with LC50 values of 36.36 μg/ml and 86.16 μg/ml, respectively.

CONCLUSION

The results indicated that the essential oil of A. macrostemon and its major constituents have good potential as a source for natural larvicides.

摘要

背景

在对来自中草药和本地野生植物的新型农用化学品进行筛选计划期间,发现干大蒜鳞茎的精油具有杀蚊幼虫活性。本研究的目的是确定精油及其主要成分化合物对库蚊幼虫,白纹伊蚊的杀幼虫活性。

方法

通过水蒸馏法从大蒜中提取精油,并通过气相色谱(GC)和气相色谱-质谱(GC-MS)进行分析。使用世界卫生组织(WHO)程序,评估了精油及其两种主要成分对第四龄白纹伊蚊幼虫的活性,24 小时后,在从 9.0-150μg/ml 的各种精油/化合物浓度下记录幼虫死亡率。

结果

大蒜精油对早期第四龄白纹伊蚊幼虫表现出杀幼虫活性,LC₅₀值为 72.86μg/ml。两种成分化合物,二甲基三硫化物和甲基丙基二硫化物对早期第四龄白纹伊蚊幼虫具有很强的杀幼虫活性,LC50 值分别为 36.36μg/ml 和 86.16μg/ml。

结论

结果表明,大蒜精油及其主要成分具有作为天然杀虫剂来源的良好潜力。

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