Departamento de Biotecnologia, Universidade Federal de Tocantins, Gurupi, TO, 77413-070, Brazil.
Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil.
Sci Rep. 2018 May 8;8(1):7215. doi: 10.1038/s41598-018-25721-0.
Although the cultivation of transgenic plants expressing toxins of Bacillus thuringiensis (Bt) represents a successful pest management strategy, the rapid evolution of resistance to Bt plants in several lepidopteran pests has threatened the sustainability of this practice. By exhibiting a favorable safety profile and allowing integration with pest management initiatives, plant essential oils have become relevant pest control alternatives. Here, we assessed the potential of essential oils extracted from a Neotropical plant, Siparuna guianensis Aublet, for improving the control and resistance management of key lepidopteran pests (i.e., Spodoptera frugiperda and Anticarsia gemmatalis). The essential oil exhibited high toxicity against both lepidopteran pest species (including an S. frugiperda strain resistant to Cry1A.105 and Cry2Ab Bt toxins). This high insecticidal activity was associated with necrotic and apoptotic effects revealed by in vitro assays with lepidopteran (but not human) cell lines. Furthermore, deficits in reproduction (e.g., egg-laying deterrence and decreased egg viability), larval development (e.g., feeding inhibition) and locomotion (e.g., individual and grouped larvae walking activities) were recorded for lepidopterans sublethally exposed to the essential oil. Thus, by similarly and efficiently controlling lepidopteran strains susceptible and resistant to Bt toxins, the S. guianensis essential oil represents a promising management tool against key lepidopteran pests.
尽管表达苏云金芽孢杆菌（Bt）毒素的转基因植物的培育是一种成功的害虫管理策略，但几种鳞翅目害虫对 Bt 植物的快速抗药性已经威胁到了这种做法的可持续性。植物精油具有良好的安全特性，并允许与害虫管理措施相结合，因此成为相关的害虫控制替代品。在这里，我们评估了从 Neotropical 植物 Siparuna guianensis Aublet 中提取的精油在改善关键鳞翅目害虫（即 Spodoptera frugiperda 和 Anticarsia gemmatalis）的控制和抗药性管理方面的潜力。该精油对两种鳞翅目害虫均具有高毒性（包括对 Cry1A.105 和 Cry2Ab Bt 毒素具有抗性的 S. frugiperda 菌株）。这种高杀虫活性与体外鳞翅目（但不是人类）细胞系检测到的坏死和凋亡作用有关。此外，对鳞翅目昆虫亚致死暴露于精油的昆虫的繁殖（例如产卵抑制和卵活力降低）、幼虫发育（例如摄食抑制）和运动（例如个体和群体幼虫的行走活动）均受到影响。因此，S. guianensis 精油通过同样有效地控制对 Bt 毒素敏感和抗性的鳞翅目昆虫品系，是一种有前途的防治关键鳞翅目害虫的管理工具。
