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来自腺相关病毒p5和p19启动子的表达受到rep蛋白的反式负调控。

Expression from the adeno-associated virus p5 and p19 promoters is negatively regulated in trans by the rep protein.

作者信息

Beaton A, Palumbo P, Berns K I

机构信息

Hearst Microbiology Research Center, Department of Microbiology, Cornell University Medical College, New York 10021.

出版信息

J Virol. 1989 Oct;63(10):4450-4. doi: 10.1128/JVI.63.10.4450-4454.1989.

DOI:10.1128/JVI.63.10.4450-4454.1989
PMID:2550677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC251068/
Abstract

The leftward two promoters of the adeno-associated virus (AAV) 2 genome were fused to reporter genes, and the constructs were used to transfect HeLa cells. The promoters functioned constitutively but were repressed in trans by the AAV rep gene product(s). The repression was relieved by adenovirus infection. Evidence which indicated an enhancer function for the inverted terminal repeat of the AAV-2 genome was also obtained.

摘要

将腺相关病毒（AAV）2基因组的两个向左启动子与报告基因融合，并使用构建体转染HeLa细胞。这些启动子组成性发挥作用，但在反式作用中被AAV rep基因产物抑制。腺病毒感染可解除这种抑制作用。还获得了表明AAV - 2基因组反向末端重复序列具有增强子功能的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f4/251068/0ac91a964b22/jvirol00077-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f4/251068/0ac6b9de49f9/jvirol00077-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f4/251068/6a2bf24186ee/jvirol00077-0344-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f4/251068/0ac91a964b22/jvirol00077-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f4/251068/0ac6b9de49f9/jvirol00077-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f4/251068/6a2bf24186ee/jvirol00077-0344-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f4/251068/0ac91a964b22/jvirol00077-0345-a.jpg

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