Srivastava A, Lusby E W, Berns K I
J Virol. 1983 Feb;45(2):555-64. doi: 10.1128/JVI.45.2.555-564.1983.
The complete nucleotide sequence of the adeno-associated virus 2 genome was determined. The single-stranded genome is 4,675 nucleotides in length and contains inverted terminal repeats of 145 nucleotides, the first 125 nucleotides of which form a palindromic sequence. Within the inverted terminal repetitions, there are two distinct sequences representing an inversion of 43 nucleotides that can exist on either terminus. The 5' and 3' termini of three major mRNA transcripts, which are present in both spliced and unspliced forms, were also mapped on the viral genome. Potential initiation and termination codons for efficient protein synthesis were identified, and genome segments were assigned that code for three major viral capsid proteins and, possibly, some as-yet-unidentified, nonstructural viral proteins.
腺相关病毒2型基因组的完整核苷酸序列已被确定。单链基因组长度为4675个核苷酸，包含145个核苷酸的反向末端重复序列，其中前125个核苷酸形成一个回文序列。在反向末端重复序列内，有两个不同的序列，代表一个43个核苷酸的倒位，可存在于任何一个末端。三种主要mRNA转录本的5'和3'末端，以剪接和未剪接形式存在，也被定位在病毒基因组上。确定了有效蛋白质合成的潜在起始和终止密码子，并分配了基因组片段，这些片段编码三种主要的病毒衣壳蛋白，以及可能一些尚未鉴定的非结构病毒蛋白。
