Early A E, Williams J G
ICRF, Clare Hall Laboratory, South Mimms, Herts, UK.
Nucleic Acids Res. 1989 Aug 25;17(16):6473-84. doi: 10.1093/nar/17.16.6473.
There has been considerable debate about the relative contributions of transcriptional and post-transcriptional mechanisms to the regulation of prespore gene expression in Dictyostelium. We have determined the DNA sequence upstream of D19, the Dictyostelium gene encoding PsA, a prespore-specific, cell surface protein of unknown function. Our analysis of gene fusions, in which D19 upstream sequences are placed adjacent to a heterologous reporter gene, indicates that transcriptional signals alone are sufficient for the correct temporal and cell-type specific expression of this gene. We also show that the 5' and 3' boundaries of the minimal sequences necessary for correct developmental regulation lie within the region 338 to 122 nucleotides upstream of the start site of transcription but that flanking sequences seem to be necessary for optimal expression.
关于转录和转录后机制对盘基网柄菌中前孢子基因表达调控的相对贡献，一直存在着大量的争论。我们已经确定了D19基因上游的DNA序列，D19是盘基网柄菌中编码PsA的基因，PsA是一种功能未知的前孢子特异性细胞表面蛋白。我们对基因融合体进行了分析，即将D19上游序列置于异源报告基因旁边，结果表明仅转录信号就足以使该基因进行正确的时间和细胞类型特异性表达。我们还表明，正确发育调控所需的最小序列的5'和3'边界位于转录起始位点上游338至122个核苷酸的区域内，但侧翼序列似乎对最佳表达是必需的。
