Casey L, Palatnik C M, Jacobson A
Dev Biol. 1983 Jan;95(1):239-43. doi: 10.1016/0012-1606(83)90023-4.
Messenger RNA half-life in vegetatively growing cells of Dictyostelium discoideum was determined using a uridine pulse-chase procedure. In these experiments, mRNA decayed in a complex fashion, and consisted of at least two major components, one with a half-life of about 50 min and a second with a half-life of about 10 hr. These results independently confirm our previous studies on the decay of mRNA extracted from actinomycin D-treated cells. Since these results were in apparent conflict with half-life determinations obtained with a combination of actinomycin D and daunomycin (J. P. Margolskee and H. F. Lodish, 1980a, Dev. Biol. 74, 37-49), we have also studied mRNA half-life in cells treated with a combination of both drugs and found that simultaneous use of both drugs leads to accelerated mRNA decay and other noticeable side effects. In light of our observations, we have suggested an alternative to conclusions drawn by others with respect to mRNA synthesis and stability in Dictyostelium development.
使用尿苷脉冲追踪程序测定了盘基网柄菌营养生长细胞中的信使核糖核酸（mRNA）半衰期。在这些实验中，mRNA以复杂的方式衰变，并且至少由两个主要成分组成，一个半衰期约为50分钟，另一个半衰期约为10小时。这些结果独立地证实了我们之前对从放线菌素D处理的细胞中提取的mRNA衰变的研究。由于这些结果与用放线菌素D和柔红霉素组合获得的半衰期测定结果明显冲突（J.P. 马戈尔斯基和H.F. 洛迪什，1980a，《发育生物学》74卷，37 - 49页），我们还研究了用这两种药物组合处理的细胞中的mRNA半衰期，发现同时使用这两种药物会导致mRNA加速衰变以及其他明显的副作用。根据我们的观察结果，我们针对其他人关于盘基网柄菌发育过程中mRNA合成和稳定性得出的结论提出了一种替代观点。
