Akimov V N, Podosokorskaia O A, Shliapnikov M G, Gal'chenko V F
Mikrobiologiia. 2013 Nov-Dec;82(6):707-14.
In situ analysis of the 16S rRNA genes form bacterial mats of five hydrothermal springs (36-58 degrees C) in the Uzon caldera (Kamchatka, Russia) was carried out using clone libraries. Eight clone libraries contained 18 dominant phylotypes (over 4-5%). In most clone libraries, the phylotype of the green sulfur bacterium Chlorobaculum sp. was among the dominant ones. The phylotypes of the green nonsulfur bacteria Chloroflexus and Roseiflexus and of purple nonsulfur bacteria Rhodoblastus, Rhodopseudomonas, and Rhodoferax were also among the dominant ones. Cyanobacteria were represented by one dominant phylotype in a single spring. Among nonphototrophic bacteria, the dominant phylotypes belonged to Sulfyrihydrogenibium sp., Geothrixsp., Acidobacterium sp., Meiothermus sp., Thiomonas sp., Thiofaba sp., and Spirochaeta sp. Three phylotypes were not identified at the genus level. Most genera of phototrophic and nonphototrophic organisms corresponding to the phylotypes from Uzon hydrotherms have been previously revealed in the hydrotherms of volcanically active regions of America, Asia, and Europe. These results indicate predominance of bacterial mats carrying out anaerobic photosynthesis in the hydrotherms of the Uzon caldera.
利用克隆文库对俄罗斯堪察加半岛乌宗火山口5个热泉（36 - 58摄氏度）的细菌垫中的16S rRNA基因进行了原位分析。8个克隆文库包含18个优势系统发育型（超过4 - 5%）。在大多数克隆文库中，绿色硫细菌绿棒菌属（Chlorobaculum sp.）的系统发育型属于优势型。绿色非硫细菌绿屈挠菌属（Chloroflexus）和玫瑰弯菌属（Roseiflexus）以及紫色非硫细菌红芽生菌属（Rhodoblastus）、红假单胞菌属（Rhodopseudomonas）和红铁菌属（Rhodoferax）的系统发育型也属于优势型。蓝细菌在一个热泉中由一个优势系统发育型代表。在非光合细菌中，优势系统发育型属于嗜硫氢菌属（Sulfyrihydrogenibium sp.）、地丝菌属（Geothrixsp.）、嗜酸菌属（Acidobacterium sp.）、栖热放线菌属（Meiothermus sp.）、硫单胞菌属（Thiomonas sp.）、硫豆菌属（Thiofaba sp.）和螺旋体属（Spirochaeta sp.）。有3个系统发育型在属水平上未被鉴定。与乌宗热泉的系统发育型相对应的大多数光合和非光合生物属此前已在美洲、亚洲和欧洲火山活跃地区的热泉中被发现。这些结果表明，乌宗火山口热泉中进行厌氧光合作用的细菌垫占优势。
