Wu-Wang C Y, Wang S L, Stevens B, Neu J
Department of Pediatrics, University of Florida College of Medicine, Gainesville 32610.
Prostaglandins Leukot Essent Fatty Acids. 1989 Jun;36(3):129-34. doi: 10.1016/0952-3278(89)90051-3.
While prostaglandins of the E series are known to affect several small intestinal functions, their cellular mechanisms are poorly understood. The purposes of our study were to determine whether receptors for PGE are present in rat small intestine and to locate and characterize the receptor binding in the subcellular fractions. Small intestinal binding of prostaglandin E1 was significantly higher than that of prostaglandin E2. Highest receptor binding for prostaglandin E1 was found in the plasma membrane fraction of isolated small intestinal enterocytes. Curvilinearity of prostaglandin E1 binding in plasma membranes upon Scatchard analysis indicated two receptor binding sites in rat small intestine. Competitive binding studies demonstrated that receptor binding was highest for prostaglandins of the E series. These studies are the first to demonstrate specific prostaglandin E1 receptors in different subcellular fractions of rat small intestine. We suggest that receptor binding of prostaglandin E may be an important initial step in the mechanism of prostaglandin-E-induced responses in the small intestine.
虽然已知E系列前列腺素会影响多种小肠功能，但其细胞机制却知之甚少。我们研究的目的是确定大鼠小肠中是否存在前列腺素E（PGE）受体，并对亚细胞组分中的受体结合进行定位和表征。前列腺素E1在小肠中的结合显著高于前列腺素E2。在分离的小肠肠细胞的质膜组分中发现前列腺素E1的受体结合最高。Scatchard分析显示质膜中前列腺素E1结合的曲线线性表明大鼠小肠中有两个受体结合位点。竞争性结合研究表明，E系列前列腺素的受体结合最高。这些研究首次在大鼠小肠的不同亚细胞组分中证明了特异性前列腺素E1受体。我们认为，前列腺素E的受体结合可能是前列腺素E诱导小肠反应机制中的一个重要初始步骤。
