Bonnard G, Vincent F, Otten L
Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France.
Plasmid. 1989 Jul;22(1):70-81. doi: 10.1016/0147-619x(89)90037-1.
We have identified a new insertion sequence, IS866, located in the auxin synthesis gene TA iaaH of Tm4, a wide host range biotype III octopine/cucumopine type Agrobacterium tumefaciens strain with two T regions on its tumor-inducing (Ti) plasmid, TA, and TB. IS866 is 2716 bp long, has inverted repeats of 27 bp with three mismatches, and generates 8-bp direct repeats upon integration. In addition to IS866, pTiTm4 carries two copies of a related element, IS867, associated with TA and TB, respectively. A systematic study of 92 virulent Agrobacterium strains has shown that among the three biotypes all octopine/cucumopine and vitopine biotype III isolates contain IS866-like elements. The various octopine/cucumopine Ti plasmids always carry IS866 and IS867 at the same position as in pTiTm4. The chromosomes of the bacteria which contain these Ti plasmids also carry IS866 and IS867 copies but in varying numbers and locations.
我们鉴定出了一个新的插入序列IS866，它位于Tm4的生长素合成基因TA iaaH中。Tm4是一种广宿主范围的生物型III章鱼碱/黄瓜碱型根癌农杆菌菌株，其致瘤（Ti）质粒TA和TB上有两个T区域。IS866长2716 bp，有27 bp的反向重复序列，其中有三个错配，整合时会产生8 bp的正向重复序列。除了IS866，pTiTm4还携带两个相关元件IS867的拷贝，分别与TA和TB相关。对92株致病根癌农杆菌菌株的系统研究表明，在三种生物型中，所有章鱼碱/黄瓜碱和维托品生物型III分离株都含有IS866样元件。各种章鱼碱/黄瓜碱Ti质粒总是在与pTiTm4相同的位置携带IS866和IS867。含有这些Ti质粒的细菌染色体也携带IS866和IS867拷贝，但数量和位置各不相同。
