在Ti质粒pTiT37自发变体的T-DNA区域中鉴定出的一个插入序列（IS）元件的核苷酸序列。
Nucleotide sequence of an insertion sequence (IS) element identified in the T-DNA region of a spontaneous variant of the Ti-plasmid pTiT37.
作者信息
Vanderleyden J, Desair J, De Meirsman C, Michiels K, Van Gool A P, Chilton M D, Jen G C
出版信息
Nucleic Acids Res. 1986 Aug 26;14(16):6699-709. doi: 10.1093/nar/14.16.6699.
Abstract
We have identified and determined the nucleotide sequence of an IS element (IS136) of Agrobacterium tumefaciens. This is the first IS element isolated and sequenced from a nopaline type Ti-plasmid. Our IS element has 32/30 bp inverted repeats with 6 mismatches, is 1,313 bp long and generates 9 bp direct repeats upon integration. IS136 has 3 main open reading frames (ORF's). Only ORF1 (159 codons) is preceded by sequences that are proposed to serve functional roles in transcriptional and translational initiation. No DNA sequence homology was found between IS136 and IS66, an IS element isolated from an octopine type Ti-plasmid.
摘要
我们已鉴定并确定了根癌土壤杆菌的一个插入序列（IS136）的核苷酸序列。这是从胭脂碱型Ti质粒中分离并测序的首个插入序列。我们的插入序列具有32/30 bp的反向重复序列，其中有6个错配，长度为1313 bp，整合时会产生9 bp的正向重复序列。IS136有3个主要的开放阅读框（ORF）。只有ORF1（159个密码子）之前的序列被认为在转录和翻译起始中起功能作用。在IS136与从章鱼碱型Ti质粒中分离出的插入序列IS66之间未发现DNA序列同源性。
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