Gaĭseniuk F Z, Driianskaia V E, Drannik G N, Rudenko M Iu, Lavrenchuk O V, Stepanova N M, Stashevskaia N V, Busygina Iu S
Lik Sprava. 2013 Sep(6):32-7.
The antiinflammatory cytokines participate in antiinfective immunity, that is why it is advisable to study their peculiarities in determination of the role in immunologic pathogenesis of pyelonephritis. Of the work is to study the levels of pro-inflammatory blood cytokines in the patients with pyelonephritis (PN), to determine the peculiarities in acute and chronic its course in children and adults. The immuno-enzymic method ELISA and the corresponding test-systems were used to study the levels of cytokines in blood. There were studied the levels of pro-inflammatory cytokines (TNF, MCP and IL-23) in blood serum of patients with acute (APN) and chronic (CPN) pyelonephritis. The analysis showed the reliable increase in levels of all studied findings in both forms of PN--both in all 173 patients and in every group--children (87) and adults (86). The MCP-1 levels in APN are reliably higher than in CPN, while in TNF-b and IL-23--do not differ. The levels of all three cytokines in blood of adults were higher than in children in APN, but TNF--in CPN as well. The high level of TNF, MCP-1 and IL-23 in blood of adults and children confirm their important role both in APN and CPN, but MCP-1 can be considered as a predictor of acute/exacerbation of chronic pyelonephritis. By the findings of the studied cytokines, more expressed immune response was noted in the adults.
抗炎细胞因子参与抗感染免疫，这就是为何研究它们在肾盂肾炎免疫发病机制中的作用特点是可取的。这项工作旨在研究肾盂肾炎（PN）患者血液中促炎细胞因子的水平，确定其在儿童和成人急性及慢性病程中的特点。采用免疫酶联法（ELISA）和相应的检测系统来研究血液中细胞因子的水平。研究了急性肾盂肾炎（APN）和慢性肾盂肾炎（CPN）患者血清中促炎细胞因子（TNF、MCP和IL - 23）的水平。分析表明，在两种形式的PN中，所有研究指标的水平在所有173例患者以及每个组（儿童87例和成人86例）中均有可靠的升高。APN中MCP - 1水平显著高于CPN，而TNF - β和IL - 23则无差异。在APN中，成人血液中所有三种细胞因子的水平均高于儿童，但在CPN中TNF也是如此。成人和儿童血液中高水平的TNF、MCP - 1和IL - 23证实了它们在APN和CPN中都具有重要作用，但MCP - 1可被视为慢性肾盂肾炎急性发作/加重的预测指标。根据所研究细胞因子的结果，在成人中观察到更明显的免疫反应。
