持续暴露于239钚α射线对Co631细胞的影响。

Effects of continuous exposure to 239Pu-alpha rays on Co631 cells.

作者信息

Rau W, Olinger H, Planas-Bohne F, Taylor D M

机构信息

Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, F.R.G.

出版信息

Sci Total Environ. 1989 Jul 15;83(3):253-62. doi: 10.1016/0048-9697(89)90097-1.

DOI:10.1016/0048-9697(89)90097-1

PMID:2551035

Abstract

Exposure of Co631 cells to 239Pu-citrate leads to continuous irradiation and results in increasing cell death which depends on dose and incubation time of the metal. The D37 is calculated to be 0.13 Gy. Plutonium-239 induces the amplification of the integrated SV40 sequences of the Co631 cells. Other cellular genes such as the alpha-actin gene were not amplified, indicating that the SV40-gene amplification is a selective process. Southern blot analysis of genomic DNA shows that not all S40 sequences are equally amplified. Excised sequences of the SV40 genome only appear after an exposure time of 4 days.

摘要

将Co631细胞暴露于柠檬酸钚-239会导致持续照射，并导致细胞死亡增加，这取决于金属的剂量和孵育时间。计算得出D37为0.13戈瑞。钚-239诱导Co631细胞整合的SV40序列扩增。其他细胞基因如α-肌动蛋白基因未被扩增，表明SV40基因扩增是一个选择性过程。基因组DNA的Southern印迹分析表明，并非所有S40序列都被同等扩增。SV40基因组的切除序列仅在暴露4天后出现。