Ziegelmüller Brigitte, Vattai Aurelia, Kost Bernd, Kuhn Christina, Hofmann Simone, Bayer Birgit, Toth Bettina, Jeschke Udo, Ditsch Nina
Department of Obstetrics and Gynaecology-Innenstadt, Ludwig-Maximilians University of Munich, Munich, Germany (BZ, AV, BK, CK, SH, UJ)
Department of Urology, Ludwig-Maximilians University of Munich, Munich, Germany (BZ)
J Histochem Cytochem. 2015 Jul;63(7):511-23. doi: 10.1369/0022155415582052. Epub 2015 Mar 26.
Thyroid hormones are essential for the maintenance of pregnancy, and a deficiency in maternal thyroid hormones has been associated with early pregnancy losses. The expression of THRα1, THRβ1 and THRα2 increases with gestational age. The aim of this study was the investigation of the protein and mRNA-levels of THR isoforms THRα1, THRα2, THRβ1 and THRβ2 in normal, spontaneous and recurrent miscarriages. The identification of THR-expressing cells in the decidua was done with double immunofluorescence. The nuclear expression of THRα1, THRα2, THRβ1 and THRβ2 is downregulated at protein level in spontaneous and recurrent miscarriages in villous trophoblast tissue. In decidual tissue, we found a significant downregulation only for THRα1 in spontaneous miscarriages. For recurrent miscarriages, THRα1 and THRβ1 were both significantly downregulated in decidual tissue. By applying HLA-G as a trophoblast marker, we found a significant co-expression only for THRβ2. The results of our study show that thyroid hormone receptors THRα1, THRα2, THRβ1 and THRβ2 are downregulated in spontaneous and recurrent miscarriages. The majority of cells expressing the thyroid hormone receptors in the decidua are decidual stromal cells.
甲状腺激素对于维持妊娠至关重要，母体甲状腺激素缺乏与早期妊娠丢失有关。THRα1、THRβ1和THRα2的表达随孕周增加。本研究的目的是调查正常、自然流产和复发性流产中THR亚型THRα1、THRα2、THRβ1和THRβ2的蛋白质和mRNA水平。采用双重免疫荧光法鉴定蜕膜中表达THR的细胞。在绒毛滋养层组织中，自发流产和复发性流产时THRα1、THRα2、THRβ1和THRβ2的核蛋白表达下调。在蜕膜组织中，我们发现仅在自然流产时THRα1有显著下调。对于复发性流产，蜕膜组织中THRα1和THRβ1均显著下调。通过应用HLA - G作为滋养层标记物，我们发现仅THRβ2有显著共表达。我们的研究结果表明，甲状腺激素受体THRα1、THRα2、THRβ1和THRβ2在自然流产和复发性流产中下调。蜕膜中表达甲状腺激素受体的大多数细胞是蜕膜基质细胞。
