Esposito Jéssica Bordotti Nobre, Esposito Breno Pannia, Azevedo Ricardo Antunes, Cruz Luciano Soares, da Silva Luzimar Campos, de Souza Silvia Ribeiro
Instituto de Botânica, Núcleo de Ecologia, 01061-970, São Paulo, Brazil.
Environ Sci Pollut Res Int. 2015 Apr;22(7):5315-24. doi: 10.1007/s11356-014-3951-z. Epub 2014 Dec 17.
This study aimed to investigate the effects of the Mn complex (Mn(III)-desferrioxamine B (MnDFB)) on oxidative stress in the Brazilian soybean cultivar Glycine max "Sambaiba" following exposure to ozone and acid rain. We determined the suitable dose of MnDFB to apply to G. max seedlings using a dose-response curve. The highest superoxide dismutase (SOD) activity and Mn content in leaves were found upon the application of 8 μM MnDFB. Thus, G. max seedlings pretreated with 8 μM MnDFB were individually exposed to ozone and acid rain simulated. Pretreatment with MnDFB reduced lipid peroxidation upon ozone exposure and increased SOD activity in leaves; it did not alter the metal content in any part of the plant. Conversely, following acid rain exposure, neither the metal content in leaves nor SOD enzyme activity were directly affected by MnDFB, unlike pH. Our findings demonstrated that exogenous MnDFB application before ozone exposure may modulate the MnSOD, Cu/ZnSOD, and FeSOD activities to combat the ROS excess in the cell. Here, we demonstrated that the applied dose of MnDFB enhances antioxidative defenses in soybean following exposure to acid rain and especially to ozone.
本研究旨在调查锰配合物（锰（III）-去铁胺B（MnDFB））对巴西大豆品种“桑巴伊巴”（Glycine max “Sambaiba”）在暴露于臭氧和酸雨环境下时氧化应激的影响。我们通过剂量反应曲线确定了适用于大豆幼苗的MnDFB剂量。在施用8 μM MnDFB时，叶片中的超氧化物歧化酶（SOD）活性和锰含量最高。因此，用8 μM MnDFB预处理的大豆幼苗分别暴露于模拟的臭氧和酸雨环境中。MnDFB预处理可降低臭氧暴露时的脂质过氧化水平，并提高叶片中的SOD活性；它不会改变植物任何部位的金属含量。相反，在酸雨暴露后，与pH值不同，MnDFB对叶片中的金属含量和SOD酶活性均无直接影响。我们的研究结果表明，在臭氧暴露前施用外源MnDFB可能会调节锰超氧化物歧化酶（MnSOD）、铜/锌超氧化物歧化酶（Cu/ZnSOD）和铁超氧化物歧化酶（FeSOD）的活性，以对抗细胞内过量的活性氧（ROS）。在此，我们证明了施用的MnDFB剂量可增强大豆在暴露于酸雨尤其是臭氧后的抗氧化防御能力。
