Gorshkova I A, Kalinovsky A I, Ilyin S G, Gorshkov B A, Stonik V A
Pacific Institute of Bioorganic Chemistry, Far East Branch of U.S.S.R. Academy of Sciences, Vladivostok.
Toxicon. 1989;27(8):937-45. doi: 10.1016/0041-0101(89)90105-0.
High-angle X-ray diffraction spectra showed that triterpene glycosides form crystalline complexes with membrane cholesterol. Electron microscopy demonstrated a decreased vesicle size, of the membrane preparation from rat brain which is enriched in Na+-K+-ATPase, by the triterpene glycosides. The Arrhenius plot was linear in the presence of triterpene glycosides. The half-width of the phosphatidylcholine N-methyl proton line in proton NMR spectra was not altered in the presence of marine glycosides. The excimer formation of pyrene, a hydrophobic fluorescent probe, was significantly decreased by triterpene glycosides. The increase of tryptophanyl residue fluorescence demonstrated a change of the Na+-K+-ATPase conformation after treatment with cytotoxic glycosides.
高角度X射线衍射光谱表明，三萜糖苷与膜胆固醇形成结晶复合物。电子显微镜显示，三萜糖苷使富含Na+-K+-ATP酶的大鼠脑细胞膜制剂的囊泡尺寸减小。在存在三萜糖苷的情况下，阿累尼乌斯图呈线性。在存在海洋糖苷的情况下，质子核磁共振光谱中磷脂酰胆碱N-甲基质子线的半高宽没有改变。三萜糖苷显著降低了疏水性荧光探针芘的准分子形成。色氨酸残基荧光的增加表明，用细胞毒性糖苷处理后，Na+-K+-ATP酶的构象发生了变化。
